All lab work will be performed under the supervision of PI Hillary Young in her lab at the University of California, Santa Barbara. Dr. Young will be responsible for performing 16S and E-coli metabarcoding for human, domestic animal, and small mammal samples collected from each of the three villages studied in the parent grant. All lab work and bioinformatic analyses will be completed by the end of the supplementary funding period ending 2024-06-30. Work from these activities will be reported in manuscripts prepared as part of the parent grant, in addition to independent papers, subject to results. Dr. Young will comply with all NIH annual and final reporting guidelines as part of this supplementary request.