Arthropods are the sole group of animals that can produce spun silk, evolving over 23 times in insects and at least six times in crustaceans. Despite silks’ prevalence in crustaceans, most crustacean silk-spinning systems have rarely been described. Marine amphipod crustaceans have likely evolved silk spinning 2 different times once in the common ancestor of corophioids and once in ampeliscids. Both groups use silk to build structures, like tubes, that aid in feeding on suspended particles in the water. The ampeliscid silk-spinning system has yet to be investigated in detail, however, my preliminary data reveals that the morphology of the ampeliscid silk-spinning system is similar to corophioids despite being distantly related. How these nearly identical silk-spinning systems evolved in divergent groups is my driving question. To investigate this, I am using a combination of long-read genomic sequencing and RNA-Seq experiments which will reveal the molecular basis of these silk-spinning systems and identify what genes are key to evolving amphipod silk. This work is foundational in the field of crustacean silk evolution and has broad applications in studying the evolution of silk across arthropods.